Mutation detection using ligase chain reaction in passivated silicon-glass microchips and microchip capillary electrophoresis.

An inexpensive and portable microchip-based platform for integrated RT-PCR and capillary electrophoresis.

We present an inexpensive, portable and integrated microfluidic instrument that is optimized to perform genetic amplification and analysis on a single sample. Biochemical reactions and analytical separations for genetic analysis are performed within tri-layered glass-PDMS microchips. The microchip itself consists of integrated pneumatically-actuated valves and pumps for fluid handling, a thin-f...

Molecular diagnostics on microfabricated electrophoretic devices: from slab gel- to capillary- to microchip-based assays for T- and B-cell lymphoproliferative disorders.

BACKGROUND Current methods for molecular-based diagnosis of disease rely heavily on modern molecular biology techniques for interrogating the genome for aberrant DNA sequences. These techniques typically include amplification of the target DNA sequences followed by separation of the amplified fragments by slab gel electrophoresis. As a result of the labor-intensive, time-consuming nature of sla...

Small-sized capillary electrophoresis with a chemiluminescence detector equipped with cross-intersection for sample injection.

been aggressively studied based on the concept of the micro total analysis system (µ-TAS). It is called microchip CE. Laser-induced fluorescence (LIF) has been most commonly used as a sensitive detection method in microchip CE. 1 However, LIF detection necessitates a laser light source or spectroscopes. An alternative sensitive detection scheme involves the use of chemiluminescence (CL). Becaus...

Poly(dimethylsiloxane) microchip for precolumn reaction and micellar electrokinetic chromatography of biogenic amines.

We have demonstrated that precolumn derivatization and capillary electrophoresis separation on a poly(dimethylsiloxane) (PDMS) microchip can be realized as efficient as those on glass microchips. In an optimized condition of micellar electrokinetic chromatography (MEKC), using 25 mM sodium borate buffer (pH 10.0) with 25 mM sodium dodecyl sulfate (SDS) and 5% v/v methanol, the electroosmotic fl...

Influence of silicon membrane interposed between glass plates on microchip capillary electrophoresis with a chemiluminescence detector.